Flow Cytometry

Applications

  • Cell cycle
  • Proliferation
  • Apoptosis
  • Soluble proteins
  • Cell surface and intracellular proteins
  • Intracellular calcium and reactive oxygen species

Instruments

BD LSR II

  • Three lasers, 11 colors
  • Solid-state 488nm coherent sapphire blue laser
    • FITC, PE, PE-TexasRed, PerCP-Cy5.5, PE-Cy7
  • Solid-state 640nm red laser
    • APC, Alexa700, APC-Cy7
  • Solid-state 405nm violet laser
    • Pacific blue, AmCyan, Qdot 605
  • Sigital acquisition using FACSDiva v6

BD FACSCalibur

  • Two lasers, four colors, sorting
  • Air-cooled argon-ion 488nm laser
    • FITC, PE, PerCP
  • Red-diode 635nm laser
    • APC
  • CellQuest and FACSComp for acquisition and analysis